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Dong, G.* ; Callegari, E.A.* ; Gloeckner, C.J. ; Ueffing, M. ; Wang, H.M.*

Prothymosin-α interacts with mutant huntingtin and suppresses its cytotoxicity in cell culture.

J. Biol. Chem. 287, 1279-1289 (2012)
DOI PMC
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Huntington disease (HD), a fatal neurodegenerative disorder, is caused by a lengthening of the polyglutamine tract in the huntingtin (Htt) protein. Despite considerable effort, thus far there is no cure or treatment available for the disorder. Using the approach of tandem affinity purification we recently discovered that prothymosin-alpha (ProT alpha), a small highly acidic protein, interacts with mutant Htt (mHtt). This was confirmed by co-immunoprecipitation and a glutathione S-transferase (GST) pull-down assay. Overexpression of ProT alpha remarkably reduced mHtt-induced cytotoxicity in both non-neuronal and neuronal cell models expressing N-terminal mHtt fragments, whereas knockdown of ProT alpha expression in the cells enhanced mHtt-caused cell death. Deletion of the central acidic domain of ProT alpha abolished not only its interaction with mHtt but also its protective effect on mHtt-caused cytotoxicity. Additionally, overexpression of ProT alpha inhibited caspase-3 activation but enhanced aggregation of mHtt. Furthermore, when added to cultured cells expressing mHtt, the purified recombinant ProT alpha protein not only entered the cells but it also significantly suppressed the mHtt-caused cytotoxicity. Taken together, these data suggest that ProT alpha might be a novel therapeutic target for treating HD and other polyglutamine expansion disorders.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Huntington’s disease; polyglutamine; huntingtin; Prothymosin-α; cell death; protein-protein interaction; aggregation; CREB-BINDING PROTEIN; NEURONAL DEATH; DISEASE; TOXICITY; NECROSIS; TRANSCRIPTION; CONFORMATION; PATHOGENESIS; AGGREGATION; UBIQUILIN
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Zeitschrift Journal of Biological Chemistry, The
Quellenangaben Band: 287, Heft: 2, Seiten: 1279-1289 Artikelnummer: , Supplement: ,
Verlag American Society for Biochemistry and Molecular Biology
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)