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Schluesche, P.* ; Stelzer, G. ; Piaia, E. ; Lamb, D.C.* ; Meisterernst, M.

NC2 mobilizes TBP on core promoter TATA boxes.

Nat. Struct. Mol. Biol. 14, 1196-1201 (2007)
Verlagsversion DOI
Open Access Gold
The general transcription factors (GTFs) of eukaryotic RNA polymerase II, in a process facilitated by regulatory and accessory factors, target promoters through synergistic interactions with core elements. The specific binding of the TATA box-binding protein (TBP) to the TATA box has led to the assumption that GTFs recognize promoters directly, producing a preinitiation complex at a defined position. Using biochemical analysis as well as biophysical single-pair Förster resonance energy transfer, we now provide evidence that negative cofactor-2 (NC2) induces dynamic conformational changes in the TBP-DNA complex that allow it to escape and return to TATA-binding mode. This can lead to movement of TBP along the DNA away from TATA.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter DNA/chemistry; Electrophoretic Mobility Shift Assay; Fluorescence Resonance Energy Tansfer;Humans; Molecular Structure; Phosphoproteins/chemistry; Phosphoproteins/physiology*; Promoter Regions; Genetic*; TATA Box* TATA-Box Binding; Protein/chemistry
Sprache englisch
Veröffentlichungsjahr 2007
HGF-Berichtsjahr 0
ISSN (print) / ISBN 1545-9993
e-ISSN 1545-9985
Zeitschrift Nature Structural & Molecular Biology
Quellenangaben Band: 14, Heft: 12, Seiten: 1196-1201 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Immunology (IMI)
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) FE 71781
DOI 10.1038/nsmb1328
Scopus ID 36849060317
Erfassungsdatum 2007-12-10
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