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Caldwell, R.B. ; Fiedler, P. ; Schoetz, U. ; Buerstedde, J.-M.

Gene Function Analysis Using the Chicken B-Cell Line DT40.

In: Gene Function Analysis. Berlin [u.a.]: Springer, 2007. 193-210 (Methods Mol. Biol. ; 408)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Quidquid agis, prudenter agas et respice finem!—Whatever you do, do it wisely and consider the goal. In consideration of that sage advice, the chicken B-cell line DT40 is an excellent model cell system to study the function of vertebrate genes. In addition to being highly amenable to gene manipulations, the recent influx of genome and gene/protein resources allows for the straightforward selection, design, and targeting of candidate genes for knockout analysis. This chapter will give a step by step standardized protocol to creating a gene knockout mutant in DT40. With careful consideration, the methods and protocols described herein can be easily modified to allow for further gene manipulations such as creating a knockin or a conditional mutant.
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Publikationstyp Artikel: Sammelbandbeitrag/Buchkapitel
Herausgeber Ochs, M.*
Schlagwörter Conditional mutant; DT40; gene function analysis; gene manipulation; genotype; homologous recombination; knockin; knockout; phenotype; targeting
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
ISBN 978-1-58829-734-1
Bandtitel Gene Function Analysis
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 408, Heft: , Seiten: 193-210 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Radiation Biology (IMS)