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Aubele, M. ; Smida, J.

Degenerate Oligonucleotide-Primed PCR.

Methods Mol. Biol. 226, 315-318 (2003)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The amount of genomic DNA available for genetic studies can often be limiting. Degenerated oligonucleotide-primed polymerase chain reaction (DOP-PCR) is an appropriate method for overcoming these limitations by efficiently performing whole genome amplification. The DOP-PCR technique is increasingly being applied for simultaneous amplification of multiple loci in target DNA using oligonucleotide primers of partially degenerate sequences. Contrary to other PCR-based general amplification methods (Alu-PCR or IRS-PCR), DOP-PCR is a species-independent technique for the amplification of small amounts of DNA.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Herausgeber Bartlett, J.M.S.* ; Stirling, D.*
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Konferenztitel Methods in Molecular Biology : PCR Protocols
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 226, Heft: , Seiten: 315-318 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pathology (PATH)