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Degenerate Oligonucleotide-Primed PCR.
Methods Mol. Biol. 226, 315-318 (2003)
The amount of genomic DNA available for genetic studies can often be limiting. Degenerated oligonucleotide-primed polymerase chain reaction (DOP-PCR) is an appropriate method for overcoming these limitations by efficiently performing whole genome amplification. The DOP-PCR technique is increasingly being applied for simultaneous amplification of multiple loci in target DNA using oligonucleotide primers of partially degenerate sequences. Contrary to other PCR-based general amplification methods (Alu-PCR or IRS-PCR), DOP-PCR is a species-independent technique for the amplification of small amounts of DNA.
Impact Factor
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0.200
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Anmerkungen
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Bartlett, J.M.S.* ; Stirling, D.*
Sprache
englisch
Veröffentlichungsjahr
2003
HGF-Berichtsjahr
0
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Methods in Molecular Biology : PCR Protocols
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 226,
Seiten: 315-318
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Pathology (PATH)
Forschungsfeld(er)
Enabling and Novel Technologies
PSP-Element(e)
FE 70332
Erfassungsdatum
2003-09-10