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Fritz, E. ; Fekete, A. ; Lintelmann, J. ; Schmitt-Kopplin, P. ; Meckenstock, R.U.

Isolation of two Pseudomonas strains producing pseudomonic acid A.

Syst. Appl. Microbiol. 32, 56-64 (2009)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Two novel Pseudomonas strains were isolated from groundwater sediment samples. The strains showed resistance against the antibiotics tetracycline, cephalothin, nisin, vancomycin. Nalidixic acid, erythromycin, lincomycin, and penicillin and grew at temperatures between 15 and 37 degrees C and pH values from 4 to 10 with a maximum at pH 7 to 10. The 16S ribosomal RNA gene sequences and the substrate spectrum of the isolates revealed that the two strains belonged to the Pseudomonas fluorescens group. The supernatants of both strains had an antibiotic effect against Gram-positive bacteria and one Gram-negative strain. The effective substance was produced under standard cultivation conditions without special inducer molecules or special medium composition. The antibiotically active compound was identified as pseudomonic acid A by off-line high performance liquid chromatography (HPLC) and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The measurement on ultra performance liquid chromatography (UPLC, UV-vis detection) confirmed the determination of pseudomonic acid A which was produced by both strains at 1.7-3.5 mg/l. Our findings indicate that the ability to produce the antibiotic pseudomonic acid A (Mupirocin) is more spread among the pseudomonads then anticipated from the only producer known so far.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Mupirocin; Pseudomonas fluorescens; Environmental isolates; FT-ICR-MS; UPLC; HPLC; Antibiotic; performance liquid-chromatography; macrolide antibiotics; avoid suicide; fluorescens; diversity; biosynthesis; frequency; community; uplc; hplc
ISSN (print) / ISBN 0723-2020
e-ISSN 1618-0984
Zeitschrift Systematic and Applied Microbiology
Quellenangaben Band: 32, Heft: 1, Seiten: 56-64 Artikelnummer: , Supplement: ,
Verlag Urban & Fischer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)
Institute of Ecological Chemistry (IOEC)
Cooperation Group Comprehensive Molecular Analytics (CMA)