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Du, T.G.* ; Jellbauer, S.* ; Müller, M. ; Schmid, M.* ; Niessing, D. ; Jansen, R.P.*

Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA.

EMBO Rep. 9, 781-787 (2008)
DOI
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Cytoplasmic localization and localized translation of messenger RNAs contribute to asymmetrical protein distribution. Recognition of localized mRNAs by RNA-binding proteins can occur in the cytoplasm or, alternatively, co- or post-transcriptionally in the nucleus. In budding yeast, mRNAs destined for localization are bound by the She2 protein before their nuclear export. Here, we show that a specific transcript, known as ASH1 mRNA, and She2 localize specifically to the nucleolus when their nuclear export is blocked. Nucleolar She2 localization is enhanced in a She2 mutant that cannot bind to RNA. A fusion protein of the amino terminus of She3 and She2 (She3N-She2) fails to enter the nucleus, but does not impair ASH1 mRNA localization. Instead, these cells fail to distribute Ash1 protein asymmetrically, which is caused by a defective translational control of ASH1 mRNA. Our results indicate that the nucleolar transit of RNA-binding proteins such as She2 is necessary for the correct assembly of translationally silenced localizing messenger ribonucleoproteins.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter mRNA localization; nucleolus; Saccharomyces cerevisiae; SHE2; translational control
ISSN (print) / ISBN 1469-221X
e-ISSN 1469-3178
Zeitschrift EMBO Reports
Quellenangaben Band: 9, Heft: 8, Seiten: 781-787 Artikelnummer: , Supplement: ,
Verlag EMBO Press
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)