Startseite
English
Erweiterte Suche
Durchblättern nach ...
Publikationen im Überblick
Ansprechpartner
Hilfe
DOI
PMC
 |
|
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Practical considerations for the analysis of ionic and neutral organic molecules with capillary electrophoresis/mass spectrometry.
Methods Mol. Biol. 384, 135-156 (2008)
This chapter presents the technique of capillary electrophoresis coupled to mass spectrometry (CE/MS). The introductory section is targeted mainly at CE/MS beginners and notes briefly the theoretical background of electrospray ionization (ESI), the most commonly used ionization mode in CE/MS. The specifics of CE/MS are described—also in comparison with more classic methods like LC/MS. Important caveats to be taken nto consideration for successful CE/MS operation are noted in the interest of avoiding pitfalls. CE/MS is illustrated with three representative examples, which might serve as starting points for more in-detail experiments: (1) partial-filling micellar elektrokinetic chromatography (MEKC) ofneutral bacterial signaling molecules (N-acylhomoserine lactones) extracted from culture supernatants, (2) capillary zone electrophoresis (CZE) of their anionic degradation products, and finally (3) CZE separation of cationic hydroxys-triazines.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten
[➜Einloggen]
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Schmitt-Kopplin, P.
Schlagwörter
capillary electrophoresis; mass spectrometry; electrospray ionization; coupling techniques; anions; cations; neutrals; N-acylhomoserine lactones; triazines
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Capillary Electrophoresis: Methods and Protocols
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 384,
Seiten: 135-156
Reihe
Methods in Molecular Biology
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Ecological Chemistry (IOEC)