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Construction and isolation of recombinant MVA.
Methods Mol. Biol. 269, 77-99 (2004)
Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA is its clear safety record, and it can be handled under biosafety level 1 conditions. Despite its replication deficiency in human and most mammalian cells, MVA provides high-level gene expression and has proven to be immunogenic when delivering heterologous antigens in animals and humans. This chapter provides state-of-the-art protocols for generation, plaque isolation, molecular characterization, as well as amplification and purification of MVA vector viruses to obtain recombinant viruses for further evaluation.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Isaacs, S.N.*
Schlagwörter
poxvirus; modified vaccinia virus Ankara (MVA); recombinant vaccine
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Vaccinia Virus and Poxvirology: Methods and Protocols
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 269,
Seiten: 77-99
Reihe
Methods in Molecular Biology
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Virology (VIRO)