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Mühlhaus, J.* ; Pütter, C.* ; Brumm, H.* ; Grallert, H. ; Illig, T. ; Scherag, S.* ; Reinehr, T.* ; Pott, W.* ; Albayrak, Ö.* ; Wang, H.J.* ; Bau, A.-M.* ; Wiegand, S.* ; Grüters, A.* ; Krude, H.* ; Hebebrand, J.* ; Hinney, A.* ; Biebermann, H.* ; Scherag, A.*

Do common variants separate between obese melanocortin-4 receptor gene mutation carriers and non-carriers? The impact of cryptic relatedness.

Horm. Res. Paediatr. 77, 358-368 (2012)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
BACKGROUND/AIMS: Genome-wide association studies revealed associations of single nucleotide polymorphisms (SNPs) flanking MC4R with body mass index variability and obesity. We genotyped 28 SNPs, covering MC4R, and searched for haplotypes discriminating between obese mutation carriers and non-carriers. METHODS: We analyzed all three-marker haplotype combinations of the 28 SNPs to discriminate between obese mutation carriers and non-carriers - overall and in functional categories for 25 different MC4R mutations: (a) 'like wild type', (b) 'partial loss of function', and (c) 'complete loss of function'. We checked for the possible impact of 'cryptic relatedness' by sensitivity analyses including only 1 randomly selected patient per mutation. RESULTS: Overall analyses revealed a haplotype of 3 SNPs downstream of the MC4R discriminating between obese mutation carriers and obese non-carriers. However, sensitivity analyses showed that the finding is most likely due to cryptic relatedness. CONCLUSION: Given a mutation prevalence of 1-5%, the sample size of 62 obese mutation carriers with overall 25 different MC4R mutations represents a unique feature of our study. Taking MC4R as an example, we demonstrate the impact of cryptic relatedness when trying to link non-coding SNPs to functionally relevant mutations. Hence, a thorough mutation screen can currently not be guided by SNP genotyping.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Obesity; MC4R; Mutations; Haplotypes; Cryptic relatedness; GENOME-WIDE ASSOCIATION; BODY-MASS INDEX; PROOPIOMELANOCORTIN-DERIVED AGONISTS; CELL-SURFACE EXPRESSION; EARLY-ONSET OBESITY; FUNCTIONAL-CHARACTERIZATION; ADULT OBESITY; PHARMACOLOGICAL CHARACTERIZATION; CHILDHOOD OBESITY; EXTREME OBESITY
ISSN (print) / ISBN 1663-2818
e-ISSN 1663-2826
Zeitschrift Hormone Research in Paediatrics
Quellenangaben Band: 77, Heft: 6, Seiten: 358-368 Artikelnummer: , Supplement: ,
Verlag Karger
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Molecular Epidemiology (AME)