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Inflammatory and oxidative stress responses of an alveolar epithelial cell line to airborne zinc oxide nanoparticles at the air-liquid interface: A comparison with conventional, submerged cell-culture conditions.
J. Biomed. Biotechnol. 2013:652632 (2013)
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DOI
PMC
The biological effects of inhalable nanoparticles have been widely studied in vitro with pulmonary cells cultured under submerged and air-liquid interface (ALI) conditions. Submerged exposures are experimentally simpler, but ALI exposures are physiologically more realistic and hence potentially biologically more meaningful. In this study, we investigated the cellular response of human alveolar epithelial-like cells (A549) to airborne agglomerates of zinc oxide (ZnO) nanoparticles at the ALI, compared it to the response under submerged culture conditions, and provided a quantitative comparison with the literature data on different types of particles and cells. For ZnO nanoparticle doses of 0.7 and 2.5 mu g ZnO/cm(2) (or 0.09 and 0.33 cm(2) ZnO/cm(2)), cell viability was not mitigated and no significant effects on the transcript levels of oxidative stress markers (HMOX1, SOD-2 and GCS) were observed. However, the transcript levels of proinflammatory markers (IL-, IL- 6, and GM-CSF) were induced to higher levels under ALI conditions.. is is consistent with the literature data and it suggests that in vitro toxicity screening of nanoparticles with ALI cell culture systems may produce less false negative results than screening with submerged cell cultures. However, the database is currently too scarce to draw a definite conclusion on this issue.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Diesel Exhaust Particles ; Ultrafine Particles ; In-vitro ; Surface-area ; Carbonaceous Particles ; Aerosol-particles ; Respiratory-tract ; Exposure ; Toxicity ; Model
ISSN (print) / ISBN
1110-7243
e-ISSN
1110-7251
Zeitschrift
Journal of Biomedicine and Biotechnology
Quellenangaben
Band: 2013,
Artikelnummer: 652632
Verlag
Hindawi
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed