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Samy, S.* ; Robinson, J.G.* ; Hays, M.D.*

An advanced LC-MS (Q-TOF) technique for the detection of amino acids in atmospheric aerosols.

Anal. Bioanal. Chem. 401, 3103-13 (2011)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Methodology for detection of native (underivatized) amino acids (AA) in atmospheric aerosols has been developed. This article describes the use of LC-MS (Q-TOF) and microwave-assisted gas phase hydrolysis for detection of free and combined amino acids in aerosols collected in a Southeastern U.S. forest environment. Accurate mass detection and the addition of isotopically labeled surrogates prior to sample preparation allows for sensitive quantitation of target AA in a complex aerosol matrix. A total of 16 native AA were detected above the reporting threshold as water-soluble free AA, with an average concentration of 22 ± 9 ng m(-3) (N = 13). Following microwave-assisted gas phase hydrolysis, the total AA concentration in the forest environment increased significantly (70 ± 35 ng m(-3)) and additional compounds (methionine, isoleucine) were detected above the reporting threshold. The ability to quantify AA in aerosol samples without derivatization reduces time-consuming preparation procedures while providing the advancement of selective mass determination for important organic nitrogen (ON) species. Details on sample preparation that eliminates the freeze-drying approach typically practiced for water removal with biological samples, and vapor phase microwave hydrolysis parameters are provided. Method application for determination of atmospheric ON is discussed.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 1618-2642
e-ISSN 1618-2650
Zeitschrift Analytical and Bioanalytical Chemistry
Quellenangaben Band: 401, Heft: 10, Seiten: 3103-13 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Heidelberg
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Cooperation Group Comprehensive Molecular Analytics (CMA)