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Ziegler, A.-G. ; Haupt, F. ; Scholz, M. ; Weininger, K. ; Wittich, S. ; Löbner, S. ; Matzke, C. ; Gezginci, C. ; Riethausen, S. ; Beyerlein, A. ; Zillmer, S. ; Amoroso, M.* ; Coles, R.* ; Powell, M.* ; Furmaniak, J.* ; Rees Smith, B.* ; Winkler, C. ; Bonifacio, E. ; Achenbach, P.

3 screen ELISA for high-throughput detection of beta cell autoantibodies in capillary blood.

Diabetes Technol. Ther. 18, 687-693 (2016)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
BACKGROUND: Testing for beta cell autoantibodies is used for wide-scale identification of early stages of type 1 diabetes. This requires suitable screening assays. We aimed to establish screening that utilized a first step assay (3 Screen) able to detect autoantibodies to the target antigens glutamic acid decarboxylase-65 (GAD), insulinoma-associated antigen 2 (IA-2), and zinc transporter 8 (ZnT8) to identify children positive for multiple beta cell autoantibodies. METHODS: An ELISA format was used where plates were coated with a mixture of recombinant GAD, IA-2, and ZnT8325W/R-dimer molecules. The performance was determined in venous blood from 686 first-degree relatives of patients with type 1 diabetes, and 200 patients at onset of type 1 diabetes, and applied as a screening assay in capillary blood from 33,639 general population children. RESULTS: The 3 Screen assay sensitivity for detecting autoantibody-positive patients at onset of type 1 diabetes was similar to that achieved by separate radiobinding assays (RBAs) for antibodies to GAD, IA-2, and ZnT8. Results in venous and capillary serum were correlated (R = 0.987). At a threshold corresponding to the 98th centile (29.1 U/mL) of all 33,639 capillary samples, the 3 Screen was positive in 123 samples with two or more RBA-positive antibodies to insulin, GAD, IA-2, or ZnT8, 146 with one antibody, and 479 that were RBA negative for beta cell autoantibodies. CONCLUSION: A 3 Screen ELISA was developed that was suitable for first step screening of multiple beta cell autoantibodies in capillary blood.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Antibody Standardization Program; Glutamic-acid Decarboxylase; Islet Autoantibodies; General-population; Diabetes Risk; Assays; Insulin; Prediction; Antigen-2; Diseases
ISSN (print) / ISBN 1520-9156
e-ISSN 1557-8593
Zeitschrift Diabetes Technology & Therapeutics
Quellenangaben Band: 18, Heft: 11, Seiten: 687-693 Artikelnummer: , Supplement: ,
Verlag Mary Ann Liebert
Verlagsort New Rochelle
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes Research Type 1 (IDF)
Institute of Diabetes and Obesity (IDO)
Institute for Pancreatic Beta Cell Research (IPI)