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A nonradioactive assay to measure production and processing of ribosomal RNA by 4sU-tagging.
Methods Mol. Biol. 1455, 121-131 (2016)
In vivo metabolic pulse labeling is a classical approach to assess production and processing of ribosomal RNA (rRNA). However, conventional labeling techniques can be indirect and require work with radioactivity. Here, we describe in detail a protocol for in vivo metabolic labeling, purification, and readout of nascent rRNA by 4-thiouridine (4sU). We propose 4sU labeling as standard nonradioactive technique for the analysis of rRNA metabolism during ribosome biogenesis.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
4-thiouridine ; Click Chemistry ; Metabolic Pulse Labeling ; Nascent Rna ; Ribosome Biogenesis ; Rrna Synthesis
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1455,
Seiten: 121-131
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Functional Epigenetics (IFE)