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Supplie, L.M.* ; Düking, T.* ; Campbell, G.* ; Díaz, F.* ; Moraes, C.T.* ; Götz, M. ; Hamprecht, B.* ; Boretius, S.* ; Mahad, D.* ; Nave, K.A.*

Respiration-deficient astrocytes survive as glycolytic cells in vivo.

J. Neurosci. 37, 4231-4242 (2017)
Verlagsversion DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Neurons and glial cells exchange energy-rich metabolites and it has been suggested, originally based on in vitro data, that astrocytes provide lactate to glutamatergic synapses ("lactate shuttle"). Here, we have studied astrocytes that lack mitochondrial respiration in vitro and in vivo A novel mouse mutant (GLAST(CreERT2):Cox10(flox/flox) ) was generated, in which the administration of tamoxifen causes mutant astrocytes to fail in the assembly of mitochondrial cytochrome c oxidase (COX). Focussing on cerebellar Bergmann glial cells that exhibit the highest rate of Cre-mediated recombination, we find a normal density of viable astrocytes even one year after tamoxifen-induced Cox10 gene targeting. Our data show that Bergmann glial cells, and presumably all astrocytes, can survive by aerobic glycolysis for an extended period of time, in the absence of glial pathology or unspecific signs of neurodegeneration.SIGNIFICANCE STATEMENTWhen astrocytes are placed into culture they import glucose and release lactate, an energy-rich metabolite readily metabolized by neurons. This observation led to the "glia-to-neuron lactate shuttle hypothesis", but in vivo evidence for this hypothesis is weak. To study astroglial energy metabolism and the directionality of lactate flux, we generated conditional Cox10 mouse mutants lacking mitochondrial respiration in astrocytes thus forcing these cells to survive by aerobic glycolysis. Here, we report that these mice are fully viable in the absence of any signs of glial or neuronal loss, suggesting that astrocytes are naturally glycolytic cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Astrocytes ; Brain Energy Metabolism ; Glycolysis ; Lactate Shuttle ; Mitochondria; Brain Energy-metabolism; Neuron Lactate Shuttle; C-oxidase Deficiency; Glucose-utilization; Aerobic Glycolysis; Mouse Model; Complex Iv; Blood-flow; Rat; Oligodendrocytes
ISSN (print) / ISBN 0270-6474
e-ISSN 1529-2401
Zeitschrift Journal of Neuroscience
Quellenangaben Band: 37, Heft: 16, Seiten: 4231-4242 Artikelnummer: , Supplement: ,
Verlag Society for Neuroscience
Verlagsort Washington
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)