Advances in metabolomics rely on quantification, investigation, optimization and streamlining new analytical appli- cations for small biomolecules. In this work a microfluidic large-scale integration chip pl atform for the characterization and use of biological proteins as sensors for metabolites is de veloped. Representatively, we selected the bacterial tran- scriptional regulator for the pyruvate dehydrogenase gene (PdhR) to tailor a synthetic assay for the metabolite pyruvate on chip [1]. PdhR belongs to a large family of transcriptional regulators, which either bind or dissociate from the DNA recognition sequence in dependence of cellular metabolite concentration. In here, this thermodynamic linked binding property of PdhR is exploited to measure pyruvate concentrations in high parallel fashion.