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Rago, L.* ; Popp, D.* ; Heiker, J.T. ; Harnisch, F.*

Electroactive microorganisms in mouse feces.

Electrochim. Acta 365:137326 (2021)
DOI
The gut microbiome is not only an indicator of different pathologies, but it also influences metabolism and overall health of the host. Recently, microorganisms inherent to the gut microbiome, such as Listeria monocytogenes, Enterococcus faecalis and Clostridium cochlearium, were demonstrated to be electroactive, i.e. to perform extracellular electron transfer (EET). To further explore the presence of electroactive microorganisms in the gut microbiome electrochemical enrichment starting from mouse feces was performed. Open circuit, abiotic and autoclaved inoculum controls were run in parallel. A maximum current density of 122±23 µA cm−2 at low coulombic efficiency ( ̴1%) was achieved. The presence of biofilms at the anode and microbial electrochemical activity with a formal potential of EET of 0.23±0.01 V vs. Ag/AgCl sat. KCl was demonstrated using fluorescence microscopy and cyclic voltammetry. The 16S rRNA gene sequencing and PCR-free Nanopore sequencing showed the enrichment and dominance of Shigella flexneri.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Electroactive Microorganisms ; Extracellular Electron Transfer (eet) ; Gut Microbiome ; Next Generation Sequencing (ngs) ; Whole Genome Sequencing (wgs); Shigella-flexneri; Electrochemical Reduction; Gut Microbiota; Genomes
ISSN (print) / ISBN 0013-4686
e-ISSN 0013-4686
Zeitschrift Electrochimica Acta
Quellenangaben Band: 365, Heft: , Seiten: , Artikelnummer: 137326 Supplement: ,
Verlag Elsevier
Verlagsort The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
Förderungen Helmholtz Association