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Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo.
STAR Protoc. 2:100636 (2021)
We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Cell Isolation ; Developmental Biology ; Flow Cytometry/mass Cytometry ; Molecular Biology ; Single Cell
e-ISSN
2666-1667
Zeitschrift
STAR Protocols
Quellenangaben
Band: 2,
Heft: 3,
Artikelnummer: 100636
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Transl. Stem Cell Research (ITS)