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Madhavan, B.K.* ; Han, Z.* ; Sickmann, A.* ; Pepperkok, R.* ; Nawroth, P.P. ; Kumar, V.*

A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions.

STAR Protoc. 2:100700 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cancer ; Cell Biology ; Microscopy ; Molecular Biology
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 2, Heft: 3, Seiten: , Artikelnummer: 100700 Supplement: ,
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed