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Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing.

STAR Protoc. 2:100805 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Isolation of nuclei tagged in specific cell types (INTACT) allows for stress-free and high-throughput analyses of cellular subpopulations. Here, we present an improved protocol for isolation of pure and high-quality GFP-labeled nuclei from frozen livers of INTACT mice, as well as protocols for downstream sequencing analyses. The adaptation to frozen tissue provides a pause point that allows sampling at multiple time points and/or phenotypic characterization of livers prior to nuclei isolation and downstream analyses. For complete details on the use of this protocol, please refer to Loft et al. (2021).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cell Biology ; Cell Isolation ; Cell Separation/fractionation ; Genomics ; Molecular Biology ; Rnaseq ; Sequencing
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 2, Heft: 3, Seiten: , Artikelnummer: 100805 Supplement: ,
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed