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Sigle, M.* ; Rohlfing, A.K.* ; Kenny, M.* ; Scheuermann, S.* ; Sun, N. ; Graeßner, U.* ; Haug, V.* ; Sudmann, J.* ; Seitz, C.M.* ; Heinzmann, D.* ; Schenke-Layland, K.* ; Maguire, P.B.* ; Walch, A.* ; Marzi, J.* ; Gawaz, M.P.*

Translating genomic tools to Raman spectroscopy analysis enables high-dimensional tissue characterization on molecular resolution.

Nat. Commun. 14, 16:5799 (2023)
DOI PMC
Creative Commons Lizenzvertrag
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Spatial transcriptomics of histological sections have revolutionized research in life sciences and enabled unprecedented insights into genetic processes involved in tissue reorganization. However, in contrast to genomic analysis, the actual biomolecular composition of the sample has fallen behind, leaving a gap of potentially highly valuable information. Raman microspectroscopy provides untargeted spatiomolecular information at high resolution, capable of filling this gap. In this study we demonstrate spatially resolved Raman “spectromics” to reveal homogeneity, heterogeneity and dynamics of cell matrix on molecular levels by repurposing state-of-the-art bioinformatic analysis tools commonly used for transcriptomic analyses. By exploring sections of murine myocardial infarction and cardiac hypertrophy, we identify myocardial subclusters when spatially approaching the pathology, and define the surrounding metabolic and cellular (immune-) landscape. Our innovative, label-free, non-invasive “spectromics” approach could therefore open perspectives for a profound characterization of histological samples, while additionally allowing the combination with consecutive downstream analyses of the very same specimen.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Myocardial-infarction; Secondary Structure; Neural-networks; Cells
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 14, Heft: 1, Seiten: 16, Artikelnummer: 5799 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical Pathology (AAP)
Förderungen BMBF
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
State Ministry of Baden Wurttemberg for Economic Affairs, Labour and Housing Construction
Deutsche Forschungsgemeinschaft
Interdisciplinary Center for Clinical Research Tuebingen (IZKF) Doctoral Program