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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Polyamines modulate mouse sperm motility.
Syst. Biol. Reprod. Med. 69, 435-449 (2023)
Polyamines are polycationic molecules which contains two or more amino groups (-NH3+) highly charged at physiological pH, and among them we found spermine, spermidine, putrescine, and cadaverine. They interact with proteins, nucleic acids, modulate Ca2+, K+, and Na+ channels, and protect sperm from oxidative stress. In this work, we evaluate the effect of spermine, spermidine, and putrescine on the total, progressive and kinematic parameters of motility, capacitation, acrosome reaction, also in presence and absence of the dbcAMP, an analogue of the cAMP, and the IBMX, a phosphodiesterase inhibitor. In addition, we evaluated the intracellular concentrations of cAMP [cAMP]i, and performed an in silico analysis between polyamines and the sAC from mouse to predict the possible interaction among them. Our results showed that all polyamines decrease drastically the total, progressive and the kinetic parameters of sperm motility, decrease the capacitation, and only spermidine and putrescine impeded the acquisition of acrosome reaction. Moreover, the effect of polyamines was attenuated but not countered by the addition of db-cAMP and IBMX, suggesting a possible inhibition of the sAC. Also, the presence of polyamines induced a decrease of the [cAMP]i, and the in silico analysis predicted a strong interaction among polyamines and the sAC. Overall, the evidence suggests that probably the polyamines interact and inhibit the activity of the sAC.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Spermatozoa ; Acrosome Reaction ; Capacitation ; Motility ; Soluble Adenylyl Cyclase; Soluble Adenylyl-cyclase; Acid-phosphatase; Capacitation; Identification; Spermatozoa; Calmodulin; Activation; Camp
ISSN (print) / ISBN
1939-6368
e-ISSN
1939-6376
Zeitschrift
Systems Biology in Reproductive Medicine
Quellenangaben
Band: 69,
Heft: 6,
Seiten: 435-449
Verlag
Taylor & Francis
Verlagsort
530 Walnut Street, Ste 850, Philadelphia, Pa 19106 Usa
Begutachtungsstatus
Peer reviewed
Institut(e)
Strategy and Digitalization (DIG)
Förderungen
Austrian Science Fund (FWF)
SNI-CONACyT
JCM
GEJG
GCC
COFAA-IPN, CAA
COFAA-IPN and of the SNI- CONACyT
SNI-CONACyT
JCM
GEJG
GCC
COFAA-IPN, CAA
COFAA-IPN and of the SNI- CONACyT