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Cota, P. ; Caliskan, Ö.S. ; Bastidas-Ponce, A. ; Jing, C. ; Jaki, J. ; Saber, L. ; Czarnecki, O. ; Taskin, D. ; Blöchinger, A. ; Kurth, T.* ; Sterr, M. ; Burtscher, I. ; Krahmer, N. ; Lickert, H. ; Bakhti, M.

Insulin regulates human pancreatic endocrine cell differentiation in vitro.

Mol. Metab. 79:101853 (2023)
DOI PMC
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
OBJECTIVE: The consequences of mutations in genes associated with monogenic forms of diabetes on pancreas development cannot be studied in a time-resolved fashion in vivo. More specifically, if recessive mutations in the insulin gene influences human pancreatic endocrine lineage formation is still an unresolved question. METHODS: To model the extremely reduced insulin levels in patients with recessive insulin gene mutations, we generated a novel knock-in H2B-Cherry reporter human induced pluripotent stem cell (iPSCs) line expressing no insulin upon differentiation to stem cell-derived (SC-) β cells in vitro. Differentiation of iPSCs into the pancreatic and endocrine lineage, combined with immunostaining, Western blotting and proteomics analysis phenotypically characterized the insulin gene deficiency in SC-islets. Furthermore, we leveraged FACS analysis and imaging to explore the impact of insulin shortage on human endocrine cell induction, endocrine cell type composition, differentiation and proliferation. RESULTS: Interestingly, insulin-deficient SC-islets exhibited low insulin receptor (IR) signaling when stimulated with glucose but displayed increased IR sensitivity upon treatment with exogenous insulin. Furthermore, insulin shortage did not alter neurogenin-3 (NGN3)-mediated endocrine lineage induction. Nevertheless, lack of insulin skewed the SC-islet cell composition with an increased number in SC-β cell formation at the expense of SC-α cells. Finally, insulin deficiency reduced the rate of SC-β cell proliferation but had no impact on the expansion of SC-α cells. CONCLUSIONS: Using iPSC disease modelling, we provided first evidence of insulin function in human pancreatic endocrine lineage formation. These findings help to better understand the phenotypic impact of recessive insulin gene mutations during pancreas development and shed light on insulin gene function beside its physiological role in blood glucose regulation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Insulin ; Endocrinogenesis ; Ipsc Differentiation ; Islet Composition ; Monogenic Diabetes ; β Cell; Beta-cells; Islet Architecture; Gene; Mutations; Growth; Proliferation; Progenitors; Conversion; Tissue; Mass
ISSN (print) / ISBN 2212-8778
e-ISSN 2212-8778
Zeitschrift Molecular Metabolism
Quellenangaben Band: 79, Heft: , Seiten: , Artikelnummer: 101853 Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Regeneration Research (IDR)
Institute of Diabetes and Obesity (IDO)
Förderungen DZD NEXT Young Talent Program
China Scholarship Council
Helmholtz Association-Initiative and Networking Fund (IVF)
Helmholtz Research School for Diabetes (HRD)
German Center for Diabetes Research (DZD e.V.)
Helmholtz Society