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Bataclan, M.* ; Leoni, C.* ; Moro, S.G.* ; Pecoraro, M.* ; Wong, E.H.* ; Heissmeyer, V. ; Monticelli, S.*

Crosstalk between Regnase-1 and -3 shapes mast cell survival and cytokine expression.

Life Sci. All. 7:e202402784 (2024)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Post-transcriptional regulation of immune-related transcripts by RNA-binding proteins (RBPs) impacts immune cell responses, including mast cell functionality. Despite their importance in immune regulation, the functional role of most RBPs remains to be understood. By manipulating the expression of specific RBPs in murine mast cells, coupled with mass spectrometry and transcriptomic analyses, we found that the Regnase family of proteins acts as a potent regulator of mast cell physiology. Specifically, Regnase-1 is required to maintain basic cell proliferation and survival, whereas both Regnase-1 and -3 cooperatively regulate the expression of inflammatory transcripts upon activation, with Tnf being a primary target in both human and mouse cells. Furthermore, Regnase-3 directly interacts with Regnase-1 in mast cells and is necessary to restrain Regnase-1 expression through the destabilization of its transcript. Overall, our study identifies protein interactors of endogenously expressed Regnase factors, characterizes the regulatory interplay between Regnase family members in mast cells, and establishes their role in the control of mast cell homeostasis and inflammatory responses.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Rna-binding Proteins; Au-rich Elements; Messenger-rna; Immune-responses; Tnf-alpha; T-cells; Roquin; Degradation; Database; Cleavage
ISSN (print) / ISBN 2575-1077
e-ISSN 2575-1077
Zeitschrift Life Science Alliance
Quellenangaben Band: 7, Heft: 8, Seiten: , Artikelnummer: e202402784 Supplement: ,
Verlag EMBO Press
Verlagsort Heidelberg
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Förderungen Aldo and Cele Dacco Foundation
Swiss National Science Foundation