Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Reduction in mitochondrial ATP synthesis mimics the effect of low glucose in depolarizing neurons from the subpostremal nucleus of the solitary tract of rats.
J. Bioenerg. Biomembr. 56, 483–493 (2024)
Neurons of the subpostremal nucleus of the solitary tract (NTS) respond to changes in extracellular glucose with alterations in membrane potential with both depolarization and hyperpolarization. From 5 mM glucose, a rapid shift to 0.5 mM glucose produces a membrane depolarization by an unknown mechanism in most neurons. However, the mechanism involved in this response needs to be known. Here, we investigated if the low glucose-induced depolarization could be mimicked by reducing ATP synthesis and possible mediators of this effect. We showed that applying the mitochondrial uncoupler CCCP (1 µM) reproduced the effects of low glucose depolarizing the membrane, generating an inward current, and decreasing membrane resistance. On the other hand, activation of AMPK did not alter these parameters. To test if low glucose and CCCP could depolarize the membrane by affecting the ionic gradient, we inhibited the electrogenic Na/K pump with 10 µM of ouabain. We observed a similar membrane depolarization but not a decrease in membrane resistance. We conclude that perfusion of neurons of the subpostremal NTS with a low glucose solution depolarizes the membrane by probably reducing intracellular ATP, but not by activating AMPK or decreasing the ionic gradient across the membrane.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Atp ; Glycemia ; Mitochondria ; Nts; Dorsal Vagal Complex; Protein-kinase; Food-intake; Hypoglycemia; Activation; Ampk; Glucoprivation; Localization; Involvement; Inhibitors
ISSN (print) / ISBN
0145-479X
e-ISSN
1573-6881
Zeitschrift
Journal of Bioenergetics and Biomembranes
Quellenangaben
Band: 56,
Seiten: 483–493
Verlag
Springer
Verlagsort
Dordrecht
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Diabetes and Obesity (IDO)
Förderungen
Fundao de Amparo Pesquisa do Estado de So Paulo