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Kriukova, E. ; Mazurenka, M. ; Marcazzan, S. ; Glasl, S. ; Quante, M.* ; Saur, D.* ; Tschurtschenthaler, M.* ; Puppels, G.J.* ; Gorpas, D. ; Ntziachristos, V.

Hybrid Raman and partial wave spectroscopy microscope for the characterization of molecular and structural alterations in tissue.

J. Biophotonics:e202400330 (2024)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
We present a hybrid Raman spectroscopy (RS) and partial wave spectroscopy (PWS) microscope for the characterization of molecular and structural tissue alterations. The PWS performance was assessed with surface roughness standards, while the Raman performance with a silicon crystal standard. We also validated the system on stomach and intestinal mouse tissues, two closely-related tissue types, and demonstrate that the addition of PWS information improves RS data classification for these tissue types from R2 = 0.892 to R2 = 0.964 (norm of residuals 0.863 and 0.497, respectively). Then, in a proof-of-concept experiment, we show that the hybrid system can detect changes in intestinal tissues harvested from a tumorigenic Villin-Cre, Apcfl/wt mouse. We discuss how the hybrid modality offers new abilities to identify the relative roles of PWS morphological features and Raman molecular fingerprints, possibly allowing for their combination to enhance the study of carcinogenesis and early cancer diagnostics in the future.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Raman Spectroscopy ; Field Cancerization ; Light‐scattering Spectroscopy ; Microscopy ; Multimodal Imaging ; Partial Wave Spectroscopy; In-vivo; Field Carcinogenesis; Risk Stratification; Cancer
ISSN (print) / ISBN 1864-063X
e-ISSN 1864-0648
Zeitschrift Journal of Biophotonics
Quellenangaben Band: , Heft: , Seiten: , Artikelnummer: e202400330 Supplement: ,
Verlag Wiley
Verlagsort Postfach 101161, 69451 Weinheim, Germany
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Förderungen European Union
European Union's Horizon 2020 research and innovation programme