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Hermant, C. ; Mourra-Díaz, D.M. ; Oomen, M.E. ; Altamirano-Pacheco, L. ; Pal, M. ; Nakatani, T. ; Torres-Padilla, M.E.

The transcription factor SRF regulates MERVL retrotransposons and gene expression during zygotic genome activation.

Genes Dev. 39, 490-509 (2025)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The regulatory circuitry of cell-specific transcriptional programs is thought to be influenced by transposable elements (TEs), whereby TEs serve as raw material for the diversification and genome-wide distribution of genetic elements that contain cis-regulatory activity. However, the transcriptional activators of TEs in relevant physiological contexts are largely unknown. Here, we undertook an evolutionary approach to identify regulators of two main families of MERVL, a major regulator of transcription during early mouse development. Using a combination of phyloregulatory, transcriptomic, and loss-of-function approaches, we demonstrate that SRF is a novel regulator of MERVL and embryonic transcription during zygotic genome activation. By resolving the phylogenetic history of two major MERVL families, we delineate the evolutionary acquisition of SRF and DUX binding sites and show that the acquisition of the SRF site precedes that of DUX. SRF contributes to embryonic transcription through the regulation of MERVLs, which in turn serve as promoters for host genes. Our work identifies new transcriptional regulators and TEs that shape the gene expression programs in early embryos and highlights the process of TE domestication via the sequential acquisition of transcription factor binding sites and coevolution with the host.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Mervl ; Mouse Embryos ; Retrotransposons ; Transcription; Serum Response Factor; Binding Microarray Data; C-fos Gene; Transposable Elements; Rna-seq; Online Database; Muerv-l; Mouse; Chromatin; Evolution
ISSN (print) / ISBN 0890-9369
e-ISSN 1549-5477
Zeitschrift Genes and Development
Quellenangaben Band: 39, Heft: 7-8, Seiten: 490-509 Artikelnummer: , Supplement: ,
Verlag Cold Spring Harbor Laboratory Press
Verlagsort 1 Bungtown Rd, Cold Spring Harbor, Ny 11724 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Epigenetics and Stem Cells (IES)
Förderungen National University of Mexico
EMBO
German Research Foundation (DFG)
Helmholtz Association
Helmholtz Association http://dx.doi.org/10.13039/501100009318