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Eckstein, Y.* ; Kessler, B.* ; Hinrichs, A.* ; Novak, I.* ; von Thaden, A.* ; Lorenzen, T.* ; Rathkolb, B. ; Scholz, A.* ; Blutke, A.* ; Koopmans, S.J.* ; Hrabě de Angelis, M. ; Christoffersen, B.* ; Wolf, E.* ; Renner, S.*

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.

STAR Protoc. 6, 37:103774 (2025)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Biotechnology And Bioengineering ; Metabolism ; Model Organisms; Pancreatic Beta-cells; C-peptide; Clearance; Model
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 6, Heft: 2, Seiten: 37, Artikelnummer: 103774 Supplement: ,
Verlag Elsevier
Verlagsort Radarweg 29, 1043 Nx Amsterdam, Netherlands
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Experimental Genetics (IEG)
Förderungen German Center for Diabetes Research (DZD)
EU FP7 Network for Initial Training (ITN) "EpiHealthNet"
Deutsche Forschungsgemeinschaft
EU Innovative Health Initiative "NHPig"