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Gschwendtner, S. ; Engel, M. ; Lueders, T. ; Buegger, F. ; Schloter, M.

Nitrogen fertilization affects bacteria utilizing plant-derived carbon in the rhizosphere of beech seedlings.

Plant Soil 407, 203-215 (2016)
Postprint Forschungsdaten DOI
Open Access Green
Background and aims: Forest ecosystems may act as sinks for or source of atmospheric CO2. While inorganic nitrogen (N) fertilization increases aboveground tree biomass, the effects on soil and rhizosphere microorganisms are less clear, indicating potentially unpredictable changes in nutrient cycling processes maintaining ecosystem functioning. Although plant-derived carbon (C) is the main C source in soils during the vegetation period, information on the response of rhizosphere bacteria assimilating rhizodeposits to increased soil N availability mainly for trees is missing. Methods: We performed a greenhouse experiment with 13C-CO2 labelled young beech seedlings grown under different N fertilization levels. DNA Stable Isotope Probing (DNA-SIP) in combination with TRFLP and pyrosequencing enabled us to identify bacteria assimilating plant-derived C and to assess the main responders phylogenetically. Results: Although above- and belowground allocation of recently fixed photosynthates remained unchanged, microbial rhizosphere community composition was clearly affected by fertilization. Besides, we found lower 13C incorporation into microbial biomass in fertilized soil. Moreover, it could be shown that only a small subset of the rhizosphere microbiome incorporated recently fixed C into its DNA, dominated by Proteobacteria (Alpha- and Betaproteobacteria) and Actinobacteria (Actinomycetales). Conclusions: Our results suggest that N fertilization may change both the diversity of bacterial communities using rhizodeposits and assimilation rates of recently fixed photosynthates. Given the close interaction of beneficial and/or deleterious microbes and plants in the rhizosphere, this could potentially have positive or negative implications for plant performance on long-term.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Dna-sip ; Fertilization ; Plant-derived Carbon ; Pyrosequencing ; Rhizosphere Microbiome; Elevated Atmospheric Co2; Norway Spruce Stand; Soil Microbial Biomass; Community Structure; Extraction Method; Ribosomal-rna; Forest Soils; Decomposition; Availability; Additions
ISSN (print) / ISBN 0032-079X
e-ISSN 1573-5036
Zeitschrift Plant and Soil
Quellenangaben Band: 407, Heft: 1-2, Seiten: 203-215 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Dordrecht
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Comparative Microbiome Analysis (COMI)
Institute of Computational Biology (ICB)
Institute of Groundwater Ecology (IGOE)
Research Unit Environmental Simulation (BIOP-EUS)