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Lueders, T. ; Dumont, M.G.* ; Bradford, L. ; Manefield, M.*

RNA-stable isotope probing: From carbon flow within key microbiota to targeted transcriptomes.

Curr. Opin. Biotechnol. 41, 83-89 (2016)
Postprint DOI PMC
Open Access Green
Stable isotope probing of RNA has enthused researchers right from its first introduction in 2002. The concept of a labelling-based detection of process-targeted microbes independent of cellular replication or growth has allowed for a much more direct handle on functionally relevant microbiota than by labelling of other biomarkers. This has led to a widespread application of the technology, and breakthroughs in our understanding of carbon flow in natural microbiomes, autotrophic and heterotrophic physiologies, microbial food webs, host-microbe interactions and environmental biotechnology. Recent studies detecting labelled mRNA demonstrate that RNA-SIP is not limited to the analysis of rRNA, but is currently developing towards an approach for accessing targeted transcriptomes. In combination with next-generation sequencing and other methodological advances, RNA-SIP will continue to deliver invaluable insights into the functioning of microbial communities.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Isopycnic Centrifugation Gradients; Anaerobic Digester Sludge; Elevated Atmospheric Co2; Ribosomal-rna; Bacterial-populations; Reducing Bacteria; Activated-sludge; Identification; Community; Acetate
ISSN (print) / ISBN 0958-1669
e-ISSN 1879-0429
Zeitschrift Current Opinion in Biotechnology
Quellenangaben Band: 41, Heft: , Seiten: 83-89 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)