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Zhu, B. ; Bradford, L. ; Huang, S. ; Szalay, A.A. ; Leix, C.* ; Weissbach, M.* ; Táncsics, A.* ; Drewes, J.E.* ; Lueders, T.

Unexpected diversity and high abundance of putative nitric oxide dismutase (Nod) genes in contaminated aquifers and wastewater treatment systems.

Appl. Environ. Microbiol. 83, DOI: 10.1128/AEM.02750-16 (2017)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Green
The oxygenic dismutation of NO into N2 and O2 has recently been suggested for the anaerobic methanotrophic Candidatus Methylomirabilis oxyfera and the alkane-oxidizing gammaproteobacterium HdN1. It represents a new pathway in microbial nitrogen cycling and is catalyzed by a putative NO dismutase (Nod). The formed O2 enables microbes to employ aerobic catabolic pathways in anoxic habitats, suggesting an ecophysiological niche space of substantial appeal for bioremediation and water treatment. However, it is still unknown whether this physiology is limited to M. oxyfera and HdN1, and whether it can be coupled to the oxidation of electron donors other than alkanes. Here, we report first insights into an unexpected diversity and remarkable abundance of nod genes in natural and engineered water systems. Phylogenetically diverse nod genes were recovered from a range of contaminated aquifers and N-removing wastewater treatment systems. Together with nod genes from M. oxyfera and HdN1, the novel environmental nod sequences formed no less than 6 well-supported phylogenetic clusters, clearly distinct from canonical NO-reductase (qNor and cNor) genes. The abundance of nod genes in the investigated samples ranged from 1.6 * 107 to 5.2 * 1010 copies g-1 wet sediment or sludge biomass, accounting for up to 10% of total bacterial 16S rRNA gene counts. In essence, NO dismutation could be a much more widespread physiology than currently perceived. Understanding the controls of this emergent microbial capacity could offer new routes for nitrogen elimination or pollutant remediation in natural and engineered water systems.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter "candidatus Methylomirabilis Oxyfera" ; Contaminated Aquifers ; Hdn1 ; Nitric Oxide (no) Dismutation ; No Dismutase ; Oxygenic Denitrification ; Wastewater Treatment; Anaerobic Methane Oxidation; Geobacillus-stearothermophilus; Complete Nitrification; Denitrifying Bacteria; Suspended Sludge; Nitrogen Removal; Nc10 Bacteria; Quantification; Degradation; Enrichment
ISSN (print) / ISBN 0099-2240
e-ISSN 1098-5336
Zeitschrift Applied and Environmental Microbiology
Quellenangaben Band: 83, Heft: 4 Seiten: , Artikelnummer: , Supplement: ,
Verlag American Society for Microbiology (ASM)
Verlagsort Washington
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)