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Dean-Ben, X.L. ; Gottschalk, S. ; Sela, G. ; Shoham, S.* ; Razansky, D.

Functional optoacoustic neuro-tomography of calcium fluxes in adult zebrafish brain in vivo.

Opt. Lett. 42, 959-962 (2017)
Postprint DOI PMC
Open Access Green
Genetically-encoded calcium indicators (GECIs) have revolutionized neuroimaging by enabling mapping of the activity of entire neuronal populations in vivo. Visualization of these powerful activity sensors has to date been limited to depth-restricted microscopic studies due to intense light scattering in the brain. We demonstrate, for the first time, in vivo real-time volumetric optoacoustic monitoring of calcium transients in adult transgenic zebrafish expressing the GCaMP5G calcium indicator. Fast changes in optoacoustic traces associated with GCaMP5G activity were detectable in the presence of other strongly absorbing endogenous chromophores, such as hemoglobin. The new functional optoacoustic neuroimaging method can visualize neural activity at penetration depths and spatio-temporal resolution scales not covered with the existing neuroimaging techniques.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0146-9592
e-ISSN 1539-4794
Zeitschrift Optics Letters
Quellenangaben Band: 42, Heft: 5, Seiten: 959-962 Artikelnummer: , Supplement: ,
Verlag Optical Society of America (OSA)
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)