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Uksa, M. ; Buegger, F. ; Gschwendtner, S. ; Lueders, T. ; Kublik, S. ; Kautz, T.* ; Athmann, M.* ; Köpke, U.* ; Charles Munch, J.* ; Schloter, M. ; Fischer, D.

Bacteria utilising plant-derived carbon in the rhizosphere of Triticum aestivum change in different depths of an arable soil.

Environ. Microbiol. Rep. 9, 729-741 (2017)
Postprint DOI PMC
Open Access Green
Root exudates shape microbial communities at the plant soil interface. Here we compared bacterial communities that utilise plant-derived carbon in the rhizosphere of wheat in different soil depths, including topsoil, as well as two subsoil layers up to 1 m depth. The experiment was performed in a green house using soil monoliths with intact soil structure taken from an agricultural field. To identify bacteria utilizing plant derived carbon, (13) C-CO2 labelling of plants was performed for two weeks at the EC50 stage, followed by stable isotope probing of extracted DNA from the rhizosphere combined with 16S rRNA gene-based amplicon sequencing. Our findings suggest substantially different bacterial key players and interaction mechanisms between plants and bacteria utilising plant-derived carbon in the rhizosphere of subsoils and topsoil. Among the three soil depths, clear differences were found in (13) C enrichment pattern across abundant operational taxonomic units (OTUs). Whereas OTUs linked to Proteobacteria were enriched in (13) C mainly in the topsoil, in both subsoil layers OTUs related to Cohnella, Paenibacillus, Flavobacterium showed a clear (13) C signal, indicating an important, so far overseen role of Firmicutes and Bacteriodetes in the subsoil rhizosphere.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Dna Stable Isotope Probing ; Rhizosphere ; Root Exudates ; Soil Microbiome ; Subsoil ; Topsoil; Microbial Communities; Sp-nov.; Wheat Rhizosphere; Flavobacterium; Diversity; Ecology; Growth; Field; Gradients; Subsoil
ISSN (print) / ISBN 1758-2229
e-ISSN 1758-2229
Zeitschrift Environmental Microbiology Reports
Quellenangaben Band: 9, Heft: 6, Seiten: 729-741 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Comparative Microbiome Analysis (COMI)
Research Unit Environmental Simulation (EUS)
Institute of Groundwater Ecology (IGOE)