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Táncsics, A.* ; Szalay, A.A. ; Farkas, M.* ; Benedek, T.* ; Szoboszlay, S.* ; Szabó, I.* ; Lueders, T.

Stable isotope probing of hypoxic toluene degradation at the Siklos aquifer reveals prominent role of Rhodocyclaceae.

FEMS Microbiol. Ecol. 94:fiy088 (2018)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The availability of oxygen is often a limiting factor for the degradation of aromatic hydrocarbons in subsurface environments. However, while both aerobic and anaerobic degraders have been intensively studied, degradation betwixt, under micro-or hypoxic conditions has rarely been addressed. It is speculated that in environments with limited, but sustained oxygen supply, such as in the vicinity of groundwater monitoring wells, hypoxic degradation may take place. A large diversity of subfamily I.2.C extradiol dioxygenase genes has been previously detected in a BTEX-contaminated aquifer in Hungary. Older literature suggests that such catabolic potentials could be associated to hypoxic degradation. Bacterial communities dominated by members of the Rhodocyclaceae were found, but the majority of the detected C23O genotypes could not be affiliated to any known bacterial degrader lineages. To address this, a stable isotope probing (SIP) incubation of site sediments with C-13(7)-toluene was performed under microoxic conditions. A combination of 16S rRNA gene amplicon sequencing and T-RFLP fingerprinting of C23O genes from SIP gradient fractions revealed the central role of degraders within the Rhodocyclaceae in hypoxic toluene degradation. The main assimilators of C-13 were identified as members of the genera Quatrionicoccus and Zoogloea, and a yet uncultured group of the Rhodocyclaceae.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Biodegradation ; Oxygen Limitation ; Dna-stable Isotope Probing ; Subfamily I.2.c Extradiol Dioxygenase (c23o) ; Groundwater ; Rhodocyclaceae; Pseudoxanthomonas-spadix Bd-a59; Single-cell Genomics; Sp Nov.; Contaminated Groundwater; Catechol 2,3-dioxygenase; Microbial Communities; Aromatic-compounds; Activated-sludge; Gen. Nov.; Bacterium
ISSN (print) / ISBN 0168-6496
e-ISSN 1574-6941
Zeitschrift FEMS Microbiology Ecology
Quellenangaben Band: 94, Heft: 6, Seiten: , Artikelnummer: fiy088 Supplement: ,
Verlag Wiley
Verlagsort Oxford
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)