PuSH - Publikationsserver des Helmholtz Zentrums München

Müller, J.C.* ; Lichtmannegger, J. ; Zischka, H. ; Sperling, M.* ; Karst, U.*

High spatial resolution LA-ICP-MS demonstrates massive liver copper depletion in Wilson disease rats upon Methanobactin treatment.

J. Trace Elem. Med. Biol. 49, 119-127 (2018)
Postprint DOI PMC
Open Access Green
Wilson disease (WD) is a rare genetic disorder of the copper metabolism leading to systemic copper accumulation, predominantly in the liver. The therapeutic approach in WD patients is the generation of a negative copper balance and the maintenance of copper homeostasis, currently by the use of copper chelators such as D-penicillamine (D-PA). However, in circumstances of delayed diagnosis, poor treatment compliance, or treatment failure, mortality is almost certain without hepatic transplantation. Moreover, even after years of D-PA treatment, high liver copper levels are present in WD patients.We have recently suggested the use of the bacterial peptide Methanobactin (MB), which has an outstanding binding affinity for copper, as potentially efficient and patient-friendly remedy against copper damage in WD. Here we substantiate these findings considerably, by demonstrating a significant removal of copper from liver samples of WD rats upon short, one week only, MB treatments. Using laser ablation-inductively coupled plasma mass spectrometry with a spatial resolution down to 4 pm, we demonstrate that only small copper hotspots remain in MB treated animal livers. We further demonstrate in WD rat liver, seven weeks after the stopped MB treatment, a lower liver copper concentration as compared to untreated control animals. Thus, MB highly efficiently depletes liver copper overload with a sustained therapeutic effect.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Elemental Bioimaging ; Laser Ablation-inductively Coupled Plasma-mass Spectrometry (la-icp-ms) ; Wilson Disease ; Methanobactin ; Chelation Therapy ; Copper; Spectrometry
ISSN (print) / ISBN 0946-672X
e-ISSN 1878-3252
Zeitschrift Journal of Trace Elements in Medicine and Biology
Quellenangaben Band: 49, Heft: , Seiten: 119-127 Artikelnummer: , Supplement: ,
Verlag Urban & Fischer
Verlagsort Office Jena, P O Box 100537, 07705 Jena, Germany
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Toxicology and Pharmacology (TOXI)