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Schneider, A.* ; Kurz, S.* ; Manske, K.* ; Janas, M.K.* ; Heikenwälder, M.* ; Misgeld, T.* ; Aichler, M. ; Weissmann, S.F.* ; Zischka, H. ; Knolle, P.* ; Wohlleber, D.*

Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.

Sci. Rep. 9:8492 (2019)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Flow-cytometry; Cultured-cells; Protein; Mechanisms; Mitophagy; Dynamics; Immunity; Disease; Model; Mavs
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Zeitschrift Scientific Reports
Quellenangaben Band: 9, Heft: 1, Seiten: , Artikelnummer: 8492 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical Pathology (AAP)
Institute of Molecular Toxicology and Pharmacology (TOXI)