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Feng, H.* ; Schorpp, K.K. ; Jin, J.* ; Yozwiak, C.E.* ; Hoffstrom, B.G.* ; Decker, A.M.* ; Rajbhandari, P.* ; Stokes, M.E.* ; Bender, H.G.* ; Csuka, J.M.* ; Upadhyayula, P.S.* ; Canoll, P.* ; Uchida, K.* ; Soni, R.K.* ; Hadian, K. ; Stockwell, B.R.*

Transferrin receptor is a specific ferroptosis marker.

Cell Rep. 30, 3411-3423.e7 (2020)
Postprint Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Ferroptosis is a type of regulated cell death driven by the iron-dependent accumulation of oxidized polyunsaturated fatty acid-containing phospholipids. There is no reliable way to selectively stain ferroptotic cells in tissue sections to characterize the extent of ferroptosis in animal models or patient samples. We address this gap by immunizing mice with membranes from lymphoma cells treated with the ferroptosis inducer piperazine erastin and screening similar to 4,750 of the resulting monoclonal antibodies generated for their ability to selectively detect cells undergoing ferroptosis. We find that one antibody, 3F3 ferroptotic membrane antibody (3F3-FMA), is effective as a selective ferroptosis-staining reagent. The antigen of 3F3-FMA is identified as the human transferrin receptor 1 protein (TfR1). We validate this finding with several additional anti-TfR1 antibodies and compare them to other potential ferroptosis-detecting reagents. We find that anti-TfR1 and anti-malondialdehyde adduct antibodies are effective at staining ferroptotic tumor cells in multiple cell culture and tissue contexts.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Biomarker ; Cancer ; Cell Death ; Ferroptosis ; Ferroptosis Marker ; Ferroptosis-specific Antibody ; Iron ; Ros ; Tissue Staining ; Transferrin Receptor; Dependent Iron Uptake; Cell-death; Endothelial-cells; Cancer-cells; Identification; Probe; Sensitivity; Metabolism; Oxidation; Biology
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 30, Heft: 10, Seiten: 3411-3423.e7 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Toxicology and Pharmacology (TOXI)