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Feng, H.* ; Schorpp, K.K. ; Jin, J.* ; Yozwiak, C.E.* ; Hoffstrom, B.G.* ; Decker, A.M.* ; Rajbhandari, P.* ; Stokes, M.E.* ; Bender, H.G.* ; Csuka, J.M.* ; Upadhyayula, P.S.* ; Canoll, P.* ; Uchida, K.* ; Soni, R.K.* ; Hadian, K. ; Stockwell, B.R.*

Transferrin receptor is a specific ferroptosis marker.

Cell Rep. 30, 3411-3423.e7 (2020)
Postprint Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Ferroptosis is a type of regulated cell death driven by the iron-dependent accumulation of oxidized polyunsaturated fatty acid-containing phospholipids. There is no reliable way to selectively stain ferroptotic cells in tissue sections to characterize the extent of ferroptosis in animal models or patient samples. We address this gap by immunizing mice with membranes from lymphoma cells treated with the ferroptosis inducer piperazine erastin and screening similar to 4,750 of the resulting monoclonal antibodies generated for their ability to selectively detect cells undergoing ferroptosis. We find that one antibody, 3F3 ferroptotic membrane antibody (3F3-FMA), is effective as a selective ferroptosis-staining reagent. The antigen of 3F3-FMA is identified as the human transferrin receptor 1 protein (TfR1). We validate this finding with several additional anti-TfR1 antibodies and compare them to other potential ferroptosis-detecting reagents. We find that anti-TfR1 and anti-malondialdehyde adduct antibodies are effective at staining ferroptotic tumor cells in multiple cell culture and tissue contexts.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Biomarker ; Cancer ; Cell Death ; Ferroptosis ; Ferroptosis Marker ; Ferroptosis-specific Antibody ; Iron ; Ros ; Tissue Staining ; Transferrin Receptor; Dependent Iron Uptake; Cell-death; Endothelial-cells; Cancer-cells; Identification; Probe; Sensitivity; Metabolism; Oxidation; Biology
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Journal Cell Reports
Quellenangaben Volume: 30, Issue: 10, Pages: 3411-3423.e7 Article Number: , Supplement: ,
Publisher Cell Press
Publishing Place 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Toxicology and Pharmacology (TOX)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505293-001
DOI 10.1016/j.celrep.2020.02.049
WOS ID WOS:000519189700018
Scopus ID 85081027380
PubMed ID 32160546
Erfassungsdatum 2020-04-17
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