PuSH - Publikationsserver des Helmholtz Zentrums München: Integrin activation enables sensitive detection of functional CD4<sup>+</sup> and CD8<sup>+</sup> T cells: Application to characterize SARS-CoV-2 immunity.

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Schöllhorn, A.* ; Schuhmacher, J.* ; Besedovsky, L.* ; Fendel, R.* ; Jensen, A.T.R.* ; Stevanović, S.* ; Lange, T.* ; Rammensee, H.G.* ; Born, J. ; Gouttefangeas, C.* ; Dimitrov, S.I.*

Integrin activation enables sensitive detection of functional CD4⁺ and CD8⁺ T cells: Application to characterize SARS-CoV-2 immunity.

Front. Immunol. 12:626308 (2021)

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We have previously shown that conformational change in the β₂-integrin is a very early activation marker that can be detected with fluorescent multimers of its ligand intercellular adhesion molecule (ICAM)-1 for rapid assessment of antigen-specific CD8⁺ T cells. In this study, we describe a modified protocol of this assay for sensitive detection of functional antigen-specific CD4⁺ T cells using a monoclonal antibody (clone m24 Ab) specific for the open, high-affinity conformation of the β₂-integrin. The kinetics of β₂-integrin activation was different on CD4⁺ and CD8⁺ T cells (several hours vs. few minutes, respectively); however, m24 Ab readily stained both cell types 4-6 h after antigen stimulation. With this protocol, we were able to monitor ex vivo effector and memory CD4⁺ and CD8⁺ T cells specific for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and hepatitis B virus (HBV) in whole blood or cryopreserved peripheral blood mononuclear cells (PBMCs) of infected or vaccinated individuals. By costaining β₂-integrin with m24 and CD154 Abs, we assessed extremely low frequencies of polyfunctional CD4⁺ T cell responses. The novel assay used in this study allows very sensitive and simultaneous screening of both CD4⁺ and CD8⁺ T cell reactivities, with versatile applicability in clinical and vaccination studies.

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PuSH - Publikationsserver des Helmholtz Zentrums München

Integrin activation enables sensitive detection of functional CD4+ and CD8+ T cells: Application to characterize SARS-CoV-2 immunity.

Integrin activation enables sensitive detection of functional CD4⁺ and CD8⁺ T cells: Application to characterize SARS-CoV-2 immunity.