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Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidy.
Nat. Commun. 12:4264 (2021)
Verlagsversion
Forschungsdaten
DOI
PMC
Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity, we have developed a single-nucleus RNA-seq2 method tailored for the comprehensive analysis of the nuclear transcriptome from frozen tissues, allowing the dissection of all cell types present in the liver, regardless of cell size or cellular fragility. We use this approach to characterize the transcriptional profile of individual hepatocytes with different levels of ploidy, and have discovered that ploidy states are associated with different metabolic potential, and gene expression in tetraploid mononucleated hepatocytes is conditioned by their position within the hepatic lobule. Our work reveals a remarkable crosstalk between gene dosage and spatial distribution of hepatocytes.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Gene-expression; Rna-seq; Transcription Factor; Human Hepatocytes; Ppar-alpha; Cell-cycle; Fibrosis; Polyploidization; Heterogeneity; Homeostasis
ISSN (print) / ISBN
2041-1723
e-ISSN
2041-1723
Zeitschrift
Nature Communications
Quellenangaben
Band: 12,
Heft: 1,
Artikelnummer: 4264
Verlag
Nature Publishing Group
Verlagsort
London
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Helmholtz Pioneer Campus (HPC)
Institute of Computational Biology (ICB)
Institute of Computational Biology (ICB)
Förderungen
Incubator grant sparse2big
Helmholtz Pioneer Campus
Helmholtz Pioneer Campus