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Muley, C.* ; Kotschi, S.* ; Bartelt, A.

Role of ubiquilins for brown adipocyte proteostasis and thermogenesis.

Front. Endocrin. 12:739021 (2021)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The acclimatization of brown adipose tissue (BAT) to sustained cold exposure requires an adaptive increase in proteasomal protein quality control. Ubiquilins represent a recently identified family of shuttle proteins with versatile functions in protein degradation, such as facilitating substrate targeting and proteasomal degradation. However, whether ubiquilins participate in brown adipocyte function has not been investigated so far. Here, we determine the role of ubiquilins for proteostasis and non-shivering thermogenesis in brown adipocytes. We found that Ubqln1, 2 and 4 are highly expressed in BAT and their expression was induced by cold and proteasomal inhibition. Surprisingly, silencing of ubiquilin gene expression (one or multiple in combinations) did not lead to aggravated ER stress or inflammation. Moreover, ubiquitin level and proteasomal activity under basal conditions were not impacted by loss of ubiquilins. Also, non-shivering thermogenesis measured by norepinephrine-induced respiration remained intact after loss of ubiquilins. In conclusion, ubiquilin proteins are highly abundant in BAT and regulated by cold, but they are dispensable for brown adipocyte proteostasis and thermogenesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Brown Adipose Tissue ; Cold Adaptation ; Proteostasis ; Thermogenesis ; Ubiquilins ; Ubiquitin-proteasome-system; Testis-specific Gene; Adipose-tissue
ISSN (print) / ISBN 1664-2392
e-ISSN 1664-2392
Zeitschrift Frontiers in Endocrinology
Quellenangaben Band: 12, Heft: , Seiten: , Artikelnummer: 739021 Supplement: ,
Verlag Frontiers
Verlagsort Lausanne
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Cancer (IDC)
Förderungen European Research Council
Deutsches Zentrum fur Herz-Kreislauf-Forschung Junior Research Group grant
Deutsche Forschungsgemeinschaft