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Arakawa, H. ; Bednar, T.* ; Wang, M.* ; Paul, K.* ; Mladenov, E.* ; Bencsik-Theilen, A.A.* ; Iliakis, G.*

Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells.

Nucleic Acids Res. 40, 2599-2610 (2012)
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In eukaryotes, the three families of ATP-dependent DNA ligases are associated with specific functions in DNA metabolism. DNA ligase I (LigI) catalyzes Okazaki-fragment ligation at the replication fork and nucleotide excision repair (NER). DNA ligase IV (LigIV) mediates repair of DNA double strand breaks (DSB) via the canonical non-homologous end-joining (NHEJ) pathway. The evolutionary younger DNA ligase III (LigIII) is restricted to higher eukaryotes and has been associated with base excision (BER) and single strand break repair (SSBR). Here, using conditional knockout strategies for LIG3 and concomitant inactivation of the LIG1 and LIG4 genes, we show that in DT40 cells LigIII efficiently supports semi-conservative DNA replication. Our observations demonstrate a high functional versatility for the evolutionary new LigIII in DNA replication and mitochondrial metabolism, and suggest the presence of an alternative pathway for Okazaki fragment ligation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter EMBRYONIC LETHALITY; DEFECTIVE NEUROGENESIS; MAMMALIAN-CELLS; EXCISION-REPAIR; GENE; NUCLEAR; RECOMBINATION; PROTEIN; SITES; MICE
Sprache englisch
Veröffentlichungsjahr 2012
Prepublished im Jahr 2011
HGF-Berichtsjahr 2011
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Zeitschrift Nucleic Acids Research
Quellenangaben Band: 40, Heft: 6, Seiten: 2599-2610 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Begutachtungsstatus Peer reviewed
Institut(e) Translational Metabolic Oncology (IDC-TMO)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Radiation Sciences
PSP-Element(e) G-501000-001
PubMed ID 22127868
DOI 10.1093/nar/gkr1024
Scopus ID 84859336816
Erfassungsdatum 2011-12-31
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