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Doryab, A. ; Schmid, O.

Towards a gold standard functional readout to characterize In Vitro lung barriers.

Eur. J. Pharm. Sci. 179:106305 (2022)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The development of biomimetic in vitro lung models as an alternative to animal studies is urgent to improve the predictability of the pharmacokinetics of potential new drugs. For pharmacokinetics studies, advanced in vitro lung models such as lung-chips should mimic a functional air-blood barrier. Unlike in vivo conditions, stem/primary cells and cell lines do not necessarily form a functional and tight barrier when cultured in vitro. Here, we explore the two gold standard techniques for monitoring barrier integrity: transepithelial electrical resistance (TEER) and permeability. We discuss the advantages and limitations of these methods, provide recommendations for methodological improvements, and we elude on possible future directions.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Apparent Permeability ; Barrier Integrity ; Biomimetic Barrier Model ; Epithelium ; Transepithelial Electrical Resistance (teer)
ISSN (print) / ISBN 0928-0987
e-ISSN 0928-0987
Zeitschrift European Journal of Pharmaceutical Sciences
Quellenangaben Band: 179, Heft: , Seiten: , Artikelnummer: 106305 Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Lung Health and Immunity (LHI)
Förderungen Bundesministerium für Bildung und Forschung