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Vos, D.Y.* ; Wijers, M.* ; Smit, M.* ; Huijkman, N.* ; Kloosterhuis, N.J.* ; Wolters, J.C.* ; Tissink, J.J. ; Pronk, A.C.M.* ; Kooijman, S.* ; Rensen, P.C.N.* ; Kuivenhoven, J.A.* ; Van De Sluis, B.*

Cargo-specific role for retriever subunit VPS26C in hepatocyte lipoprotein receptor recycling to control postprandial triglyceride-rich lipoproteins.

Arterioscler. Thromb. Vasc. Biol. 43, e29-e45 (2022)
Postprint DOI PMC
Open Access Green
BACKGROUND: The coiled-coil domain containing 22/coiled-coil domain containing 93/copper metabolism MURR1 domains (CCC) complex is required for the transport of low-density lipoprotein receptor (LDLR) and LRP1 (LDLR-related protein 1) from endosomes to the cell surface of hepatocytes. Impaired functioning of hepatocytic CCC causes hypercholesterolemia in mice, dogs, and humans. Retriever, a protein complex consisting of subunits VPS26C, VPS35L, and VPS29, is associated with CCC, but its role in endosomal lipoprotein receptor transport is unclear. We here investigated the contribution of retriever to hepatocytic lipoprotein receptor recycling and plasma lipids regulation. METHODS: Using somatic CRISPR/Cas9 gene editing, we generated liver-specific VPS35L or VPS26C-deficient mice. We determined total and surface levels of LDLR and LRP1 and plasma lipids. In addition, we studied the protein levels and composition of CCC and retriever. RESULTS: Hepatocyte VPS35L deficiency reduced VPS26C levels but had minimal impact on CCC composition. VPS35L deletion decreased hepatocytic surface expression of LDLR and LRP1, accompanied by a 21% increase in plasma cholesterol levels. Hepatic VPS26C ablation affected neither levels of VPS35L and CCC subunits, nor plasma lipid concentrations. However, VPS26C deficiency increased hepatic LDLR protein levels by 2-fold, probably compensating for reduced LRP1 functioning, as we showed in VPS26C-deficient hepatoma cells. Upon PCSK9 (proprotein convertase subtilisin/kexin type 9)-mediated LDLR elimination, VPS26C ablation delayed postprandial triglyceride clearance and increased plasma TG levels by 26%. CONCLUSIONS: Our study suggests that VPS35L is shared between retriever and CCC to facilitate LDLR and LRP1 transport from endosomes to the cell surface. Conversely, retriever subunit VPS26C selectively transports LRP1, but not LDLR, and thereby may control hepatic uptake of postprandial TG-rich lipoprotein remnants.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Copper ; Endosomes ; Gene Editing ; Hepatocyte ; Liver; Recessive Intellectual Disability; Wash Complex; Ldl Receptor; Multiprotein Complex; Apolipoprotein-e; Mice; Atherosclerosis; Endocytosis; Clearance; Lipase
ISSN (print) / ISBN 1079-5642
e-ISSN 1524-4636
Zeitschrift Arteriosclerosis, Thrombosis, and Vascular Biology
Quellenangaben Band: 43, Heft: 1, Seiten: e29-e45 Artikelnummer: , Supplement: ,
Verlag Lippincott Williams & Wilkins
Verlagsort Two Commerce Sq, 2001 Market St, Philadelphia, Pa 19103 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Cancer (IDC)
Förderungen Marie Curie Actions (MSCA)
De Cock-Hadders Foundation
Groningen University Institute for Drug Exploration (GUIDE)
Royal Netherlands Academy of Sciences
Netherlands Organization for Health Research and Development
Dutch Federation of University Medical Centers
Dutch Heart Foundation
European Union (Marie Sklodowska-Curie Actions Innovative Training Network (MSCA-ITN) 2020