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Darwish, E.* ; Ghosh, R.* ; Bentzer, J.* ; Tsardakas Renhuldt, N.* ; Proux-Wera, E.* ; Kamal, N. ; Spannagl, M. ; Hause, B.* ; Sirijovski, N.* ; Van Aken, O.*

The dynamics of touch-responsive gene expression in cereals.

Plant J. 116, 282-302 (2023)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Wind, rain, herbivores, obstacles, neighbouring plants, etc. provide important mechanical cues to steer plant growth and survival. Mechanostimulation to stimulate yield and stress resistance of crops is of significant research interest, yet a molecular understanding of transcriptional responses to touch is largely absent in cereals. To address this, we performed whole-genome transcriptomics following mechanostimulation of wheat, barley, and the recent genome-sequenced oat. The largest transcriptome changes occurred ±25 min after touching, with most of the genes being upregulated. While most genes returned to basal expression level by 1-2 h in oat, many genes retained high expression even 4 h post-treatment in barley and wheat. Functional categories such as transcription factors, kinases, phytohormones, and Ca2+ regulation were affected. In addition, cell wall-related genes involved in (hemi)cellulose, lignin, suberin, and callose biosynthesis were touch-responsive, providing molecular insight into mechanically induced changes in cell wall composition. Furthermore, several cereal-specific transcriptomic footprints were identified that were not observed in Arabidopsis. In oat and barley, we found evidence for systemic spreading of touch-induced signalling. Finally, we provide evidence that both the jasmonic acid-dependent and the jasmonic acid-independent pathways underlie touch-signalling in cereals, providing a detailed framework and marker genes for further study of (a)biotic stress responses in cereals.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Arabidopsis ; Barley ; Cereals ; Mechanical Stimulation ; Oat ; Touch Response ; Wheat; Mechanosensitive Channel; Mechanical Stimulation; Transcription Factors; Arabidopsis-thaliana; Plant-response; Quantification; Mitochondrial; Calmodulin; Distinct; Lignin
ISSN (print) / ISBN 0960-7412
e-ISSN 1365-313X
Zeitschrift Plant Journal, The
Quellenangaben Band: 116, Heft: 1, Seiten: 282-302 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort 111 River St, Hoboken 07030-5774, Nj Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Plant Genome and Systems Biology (PGSB)
Förderungen Crafoord Foundation
Carl Trygger Foundation
Carl Tesdorpf Stiftelse
NovoNordiskFonden
Sven and Lilly Lawski Foundation
Royal Society
Royal Physiographic Society of Lund
National Bioinformatics Infrastructure Sweden
Swedish Research Council (Vetenskapsradet)
Knut and Alice Wallenberg Foundation as part of the National Bioinformatics Infrastructure Sweden at SciLifeLab
Stiftelsen for Strategisk Forskning (SSF)
ScanOats Industrial Research Center: Lund University
Research Institute of Sweden (RISE)
Swedish University of Agricultural Sciences (SLU, Alnarp)
Lantmannen ek for
Oatly AB
Swedish Oat Fiber AB
Swedish Research Council
Knut and Alice Wallenberg Foundation