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The dynamics of touch-responsive gene expression in cereals.
Plant J. 116, 282-302 (2023)
Wind, rain, herbivores, obstacles, neighbouring plants, etc. provide important mechanical cues to steer plant growth and survival. Mechanostimulation to stimulate yield and stress resistance of crops is of significant research interest, yet a molecular understanding of transcriptional responses to touch is largely absent in cereals. To address this, we performed whole-genome transcriptomics following mechanostimulation of wheat, barley, and the recent genome-sequenced oat. The largest transcriptome changes occurred ±25 min after touching, with most of the genes being upregulated. While most genes returned to basal expression level by 1-2 h in oat, many genes retained high expression even 4 h post-treatment in barley and wheat. Functional categories such as transcription factors, kinases, phytohormones, and Ca2+ regulation were affected. In addition, cell wall-related genes involved in (hemi)cellulose, lignin, suberin, and callose biosynthesis were touch-responsive, providing molecular insight into mechanically induced changes in cell wall composition. Furthermore, several cereal-specific transcriptomic footprints were identified that were not observed in Arabidopsis. In oat and barley, we found evidence for systemic spreading of touch-induced signalling. Finally, we provide evidence that both the jasmonic acid-dependent and the jasmonic acid-independent pathways underlie touch-signalling in cereals, providing a detailed framework and marker genes for further study of (a)biotic stress responses in cereals.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Arabidopsis ; Barley ; Cereals ; Mechanical Stimulation ; Oat ; Touch Response ; Wheat; Mechanosensitive Channel; Mechanical Stimulation; Transcription Factors; Arabidopsis-thaliana; Plant-response; Quantification; Mitochondrial; Calmodulin; Distinct; Lignin
ISSN (print) / ISBN
0960-7412
e-ISSN
1365-313X
Journal
Plant Journal, The
Quellenangaben
Volume: 116,
Issue: 1,
Pages: 282-302
Publisher
Wiley
Publishing Place
111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Grants
Crafoord Foundation
Carl Trygger Foundation
Carl Tesdorpf Stiftelse
NovoNordiskFonden
Sven and Lilly Lawski Foundation
Royal Society
Royal Physiographic Society of Lund
National Bioinformatics Infrastructure Sweden
Swedish Research Council (Vetenskapsradet)
Knut and Alice Wallenberg Foundation as part of the National Bioinformatics Infrastructure Sweden at SciLifeLab
Stiftelsen for Strategisk Forskning (SSF)
ScanOats Industrial Research Center: Lund University
Research Institute of Sweden (RISE)
Swedish University of Agricultural Sciences (SLU, Alnarp)
Lantmannen ek for
Oatly AB
Swedish Oat Fiber AB
Swedish Research Council
Knut and Alice Wallenberg Foundation
Carl Trygger Foundation
Carl Tesdorpf Stiftelse
NovoNordiskFonden
Sven and Lilly Lawski Foundation
Royal Society
Royal Physiographic Society of Lund
National Bioinformatics Infrastructure Sweden
Swedish Research Council (Vetenskapsradet)
Knut and Alice Wallenberg Foundation as part of the National Bioinformatics Infrastructure Sweden at SciLifeLab
Stiftelsen for Strategisk Forskning (SSF)
ScanOats Industrial Research Center: Lund University
Research Institute of Sweden (RISE)
Swedish University of Agricultural Sciences (SLU, Alnarp)
Lantmannen ek for
Oatly AB
Swedish Oat Fiber AB
Swedish Research Council
Knut and Alice Wallenberg Foundation