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Alahmad, A.* ; Harir, M. ; Fochesato, S.* ; Tulumello, J.* ; Walker, A. ; Barakat, M.* ; Ndour, P.M.S.* ; Schmitt-Kopplin, P. ; Cournac, L.* ; Laplaze, L.* ; Heulin, T.* ; Achouak, W.*

Unraveling the interplay between root exudates, microbiota, and rhizosheath formation in pearl millet.

Microbiome 12:1 (2024)
DOI PMC
Creative Commons Lizenzvertrag
BACKGROUND: The rhizosheath, a cohesive soil layer firmly adhering to plant roots, plays a vital role in facilitating water and mineral uptake. In pearl millet, rhizosheath formation is genetically controlled and influenced by root exudates. Here, we investigated the impact of root exudates on the microbiota composition, interactions, and assembly processes, and rhizosheath structure in pearl millet using four distinct lines with contrasting soil aggregation abilities. RESULTS: Utilizing 16S rRNA gene and ITS metabarcoding for microbiota profiling, coupled with FTICR-MS metabonomic analysis of metabolite composition in distinct plant compartments and root exudates, we revealed substantial disparities in microbial diversity and interaction networks. The ß-NTI analysis highlighted bacterial rhizosphere turnover driven primarily by deterministic processes, showcasing prevalent homogeneous selection in root tissue (RT) and root-adhering soil (RAS). Conversely, fungal communities were more influenced by stochastic processes. In bulk soil assembly, a combination of deterministic and stochastic mechanisms shapes composition, with deterministic factors exerting a more pronounced role. Metabolic profiles across shoots, RT, and RAS in different pearl millet lines mirrored their soil aggregation levels, emphasizing the impact of inherent plant traits on microbiota composition and unique metabolic profiles in RT and exudates. Notably, exclusive presence of antimicrobial compounds, including DIMBOA and H-DIMBOA, emerged in root exudates and RT of low aggregation lines. CONCLUSIONS: This research underscores the pivotal influence of root exudates in shaping the root-associated microbiota composition across pearl millet lines, entwined with their soil aggregation capacities. These findings underscore the interconnectedness of root exudates and microbiota, which jointly shape rhizosheath structure, deepening insights into soil-plant-microbe interactions and ecological processes shaping rhizosphere microbial communities. Deciphering plant-microbe interactions and their contribution to soil aggregation and microbiota dynamics holds promise for the advancement of sustainable agricultural strategies. Video Abstract.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Exudates ; Metabonomics ; Microbiota ; Pearl Millet ; Soil Aggregation; Rhizosphere Microbiome; Chemical-composition; System Architecture; Soil Aggregation; Plant; Complex; Metabolomics; Efficiency; Strategies; Induction
ISSN (print) / ISBN 2049-2618
e-ISSN 2049-2618
Zeitschrift Microbiome
Quellenangaben Band: 12, Heft: 1 Seiten: , Artikelnummer: 1 Supplement: ,
Verlag BioMed Central
Verlagsort London
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical BioGeoChemistry (BGC)
Förderungen French Agence Nationale de la Recherche