PuSH - Publikationsserver des Helmholtz Zentrums München

Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography.

Curr. Opin. Cell Biol. 88:102356 (2024)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Cryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic reconstructions and the serial lift-out sample preparation procedure will soon make it possible to perform high-resolution structural biology in a whole new range of samples, from multicellular organisms to organoids and tissues.
Impact Factor
Scopus SNIP
Altmetric
6.000
0.000
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Schlagwörter Actin Filaments ; Automation ; Cryo-electron Tomography ; Deep Learning-based Data Analysis ; Lift-out ; Molecular Sociology ; Tomogram Acquisition; Organization; Resolution
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 0955-0674
e-ISSN 1879-0410
Quellenangaben Band: 88, Heft: , Seiten: , Artikelnummer: 102356 Supplement: ,
Verlag Elsevier
Verlagsort Kidlington
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Pioneer Campus
PSP-Element(e) G-510008-001
Förderungen Deutsche Forschungsgemeinschaft (DFG)
PubMed ID 38608425
Erfassungsdatum 2024-06-04