PuSH - Publikationsserver des Helmholtz Zentrums München

Schneider, J. ; Jasnin, M.

Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography.

Curr. Opin. Cell Biol. 88:102356 (2024)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Cryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic reconstructions and the serial lift-out sample preparation procedure will soon make it possible to perform high-resolution structural biology in a whole new range of samples, from multicellular organisms to organoids and tissues.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter Actin Filaments ; Automation ; Cryo-electron Tomography ; Deep Learning-based Data Analysis ; Lift-out ; Molecular Sociology ; Tomogram Acquisition; Organization; Resolution
ISSN (print) / ISBN 0955-0674
e-ISSN 1879-0410
Zeitschrift Current Opinion in Cell Biology
Quellenangaben Band: 88, Heft: , Seiten: , Artikelnummer: 102356 Supplement: ,
Verlag Elsevier
Verlagsort Kidlington
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
Förderungen Deutsche Forschungsgemeinschaft (DFG)