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Protocol to generate a microfluidic vessels-on-chip platform using human pluripotent stem cell-derived endothelial cells.

STAR Protoc. 5:103300 (2024)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Here, we present a protocol for producing a microfluidic vessel-on-chip platform using human pluripotent stem cell-derived endothelial cells (SC-ECs). We describe steps for manufacturing the 3D-printed chip, cell culturing to generate SC-ECs, hydrogel patterning, and the formation and cultivation of barrier-forming vessels. We then detail procedures for the retrieval of cells and media from the open microfluidic chip platform to enable multi-omics analysis. For complete details on the use and execution of this protocol, please refer to Marder et al.1.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Biotechnology And Bioengineering ; Cell Culture ; Stem Cells ; Tissue Engineering
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 5, Heft: 3, Seiten: , Artikelnummer: 103300 Supplement: ,
Verlag Elsevier
Verlagsort Radarweg 29, 1043 Nx Amsterdam, Netherlands
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
Förderungen ERC Consolidator Grant
BMBF
Helmholtz Pioneer Campus