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Shimakawa, G.* ; Demulder, M.* ; Flori, S.* ; Kawamoto, A.* ; Tsuji, Y.* ; Nawaly, H.* ; Tanaka, A.* ; Tohda, R.* ; Ota, T.* ; Matsui, H.* ; Morishima, N.* ; Okubo, R.* ; Wietrzynski, W.* ; Lamm, L. ; Righetto, R.* ; Uwizeye, C.* ; Gallet, B.* ; Jouneau, P.H.* ; Gerle, C.* ; Kurisu, G.* ; Finazzi, G.* ; Engel, B.D.* ; Matsuda, Y.*

Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation.

Cell 187, 5919-5934 (2024)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Pyrenoids are subcompartments of algal chloroplasts that increase the efficiency of Rubisco-driven CO2 fixation. Diatoms fix up to 20% of global CO2, but their pyrenoids remain poorly characterized. Here, we used in vivo photo-crosslinking to identify pyrenoid shell (PyShell) proteins, which we localized to the pyrenoid periphery of model pennate and centric diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana. In situ cryo-electron tomography revealed that pyrenoids of both diatom species are encased in a lattice-like protein sheath. Single-particle cryo-EM yielded a 2.4-Å-resolution structure of an in vitro TpPyShell1 lattice, which showed how protein subunits interlock. T. pseudonana TpPyShell1/2 knockout mutants had no PyShell sheath, altered pyrenoid morphology, and a high-CO2 requiring phenotype, with reduced photosynthetic efficiency and impaired growth under standard atmospheric conditions. The structure and function of the diatom PyShell provide a molecular view of how CO2 is assimilated in the ocean, a critical ecosystem undergoing rapid change.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Co(2)-concentrating Mechanism ; Carbon Fixation ; Chloroplast ; Cryo-em ; Cryo-et ; Diatom ; Focused Ion Beam ; In vitro Reconstitution ; Mass Spectrometry ; Photosynthesis ; Pyrenoid
ISSN (print) / ISBN 0092-8674
e-ISSN 1097-4172
Zeitschrift Cell
Quellenangaben Band: 187, Heft: 21, Seiten: 5919-5934 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort Cambridge, Mass.
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Artifical Intelligence Cooperation Unit (HAICU)