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Shimakawa, G.* ; Demulder, M.* ; Flori, S.* ; Kawamoto, A.* ; Tsuji, Y.* ; Nawaly, H.* ; Tanaka, A.* ; Tohda, R.* ; Ota, T.* ; Matsui, H.* ; Morishima, N.* ; Okubo, R.* ; Wietrzynski, W.* ; Lamm, L. ; Righetto, R.* ; Uwizeye, C.* ; Gallet, B.* ; Jouneau, P.H.* ; Gerle, C.* ; Kurisu, G.* ; Finazzi, G.* ; Engel, B.D.* ; Matsuda, Y.*

Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation.

Cell 187, 5919-5934 (2024)
Publ. Version/Full Text DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Pyrenoids are subcompartments of algal chloroplasts that increase the efficiency of Rubisco-driven CO2 fixation. Diatoms fix up to 20% of global CO2, but their pyrenoids remain poorly characterized. Here, we used in vivo photo-crosslinking to identify pyrenoid shell (PyShell) proteins, which we localized to the pyrenoid periphery of model pennate and centric diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana. In situ cryo-electron tomography revealed that pyrenoids of both diatom species are encased in a lattice-like protein sheath. Single-particle cryo-EM yielded a 2.4-Å-resolution structure of an in vitro TpPyShell1 lattice, which showed how protein subunits interlock. T. pseudonana TpPyShell1/2 knockout mutants had no PyShell sheath, altered pyrenoid morphology, and a high-CO2 requiring phenotype, with reduced photosynthetic efficiency and impaired growth under standard atmospheric conditions. The structure and function of the diatom PyShell provide a molecular view of how CO2 is assimilated in the ocean, a critical ecosystem undergoing rapid change.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Co(2)-concentrating Mechanism ; Carbon Fixation ; Chloroplast ; Cryo-em ; Cryo-et ; Diatom ; Focused Ion Beam ; In vitro Reconstitution ; Mass Spectrometry ; Photosynthesis ; Pyrenoid; Carbonic-anhydrase; Chlamydomonas-reinhardtii; Co2-concentrating Mechanism; Beta-carboxysome; Ribulose-1,5-bisphosphate Carboxylase/oxygenase; Immunocytochemical Localization; Phaeodactylum-tricornutum; Concentrating Mechanisms; Cryoelectron Tomography; Photosynthesis
ISSN (print) / ISBN 0092-8674
e-ISSN 1097-4172
Journal Cell
Quellenangaben Volume: 187, Issue: 21, Pages: 5919-5934 Article Number: , Supplement: ,
Publisher Cell Press
Publishing Place Cambridge, Mass.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Helmholtz Artifical Intelligence Cooperation Unit (HAICU)
Grants EMBO
Alexander Von Humboldt Foundation
Royal Society
EMBO short-term fellowship
Swiss State Secretariat for Education, Research and Innovation
European Research Council (ERC)
JST CREST ''Cell dynamics''
Japan Society for the Promotion of Science (JSPS) KAKENHI
Boehringer Ingelheim Fonds fellowship